Table 1 The primer sequences for RT-qPCR
Gene type | Primer sequence |
|---|---|
MST | Forward: 5′-CCTCCCACATTCCGAAAACCA-3′ |
|  | Reverse: 5′-GCACTCCTGACAAATGGGTG-3′ |
MST2 | Forward: 5′-AGGAACA-GCAACGAGAATTGG-3′ |
|  | Reverse: 5′-CCCCTTCACTCATCGTGCTT-3′ |
LATS1 | Forward: 5′-AATTTGGGACGCATCATAAAGCC-3′ |
|  | Reverse: 5′-TCGTCGAGGATCTTGGTAACTC-3′ |
LATS2 | Forward: 5′-GCTTCATCCACCGAGACATCAA-3′ |
|  | Reverse: 5′-CGACAGTTAGACACATCATCCCAGA-3′ |
YAP1 | Forward: 5′-CCTGATGGATGGGAACAAGC-3′ |
|  | Reverse:: 5′-GCACTCTGACTGATTCTCTGG-3′ |
TAZ | Forward: 5′-ACGTCCTTCCTAACAGTCC-3′ |
|  | Reverse: 5′-TGCCTGACTCTTCAGATGC-3′ |
TEAD1 | Forward: 5′-CGCCTTCTTCCTCGTCAA-3′ |
|  | Reverse: 5′-TCGCATACTCCGTCTCTAC-3′ |
TEAD2 | Forward: 5′-CCACATGCCTTCTTCCTCGTCAA-3′ |
|  | Reverse: 5′-CCGTCTCCACCTTCTCTACCACTT-3′ |
TEAD3 | Forward: 5′-TCGGCAAGCAGGTGGTAGAGAAG-3′ |
|  | Reverse: 5′-CAGGCAGGTGTGTGGAGGATGT-3′ |
TEAD4 | Forward: 5′-CTGACGGAGGAAGGCAAGATGTATG-3′ |
|  | Reverse: 5′-ACGGGCAAGCACCTGGATGT-3′ |
GAPDH | Forward: 5′-CCTTCCGTGTTCCTACCCC-3′ |
|  | Reverse: 5′-GCCCAAGATGCCCTTCAGT-3′ |