Fig. 3

The results of tandem mass spectrometry showing the increased levels of medium and long-chain acylcarnitine. A MTT method was used to detect the survival rate of H9c2 after PA (0.01, 0.03, 0.05, 0.2, 0.3, 0.5, 0.6, 0.8, 1.0 mM) treatment for 24 h to verify the virulence of PA (n = 6). B Measurement of glucose uptake using 2-NBDG (n = 6). C The expressions of p-AKT, AKT in each group (n = 6). D The analysis results of p-Akt and Akt expression (n = 6). E Tandem mass spectrometry analysis was used to analyze the cells (n = 7, data is presented with a median and compared using the Wilcoxon signed-rank test between groups). F The survival rate was analyzed using MTT assay for H9c2 after C14 (5,10,20,25,30,50,70,85,100 μM) treatment for 24 h, to verify the virulence of C14 (n = 6). The rest of the data is represented as means ± SD, where two-way ANOVA followed by Tukey’s post hoc test was used. *P < 0.05, **P < 0.01, and ***P < 0.001