Fig. 4

C14 caused the accumulation of lipids in cardiomyocytes and induced the pro-inflammatory mediators to increase the cell apoptosis. A ORO staining was used to observe the lipid deposition (n = 6, Scale bar = 50 μm, 100 μm). B Quantitative analysis of intracellular lipid droplets. C The expressions of TNFα, IL-1, and Cleaved-caspase3 in control, PA, and C14 group (n = 6), as detected using western blotting. D The results of TNFα, IL-1, and Cleaved-caspase3 expression. E The free fatty acids of H9c2 cells in each group was measured (n = 6) and compared with the treated and control group. F The data revealed positive expression of TNFα and cleaved-caspase 3 in H9c2. (G-H) Immunofluorescence staining results showing the levels of TNF-α and Cleaved-caspase3 in H9c2 (n = 6, Scale bar = 100 μm). Data are represented as means ± SD, where two-way ANOVA followed by Tukey’s post hoc test employed. *P < 0.05, **P < 0.01, and ***P < 0.001