Fig. 5

C14 can inhibit the AMPK/ACC/CPT1-b signaling pathway and causes lipid accumulation. A The expressions of P-AMPK, AMPK, P-ACC, ACC, and CPT1-b in each group (n = 6), as detected by the western blotting. B-D Quantification of p-AMPK, AMPK, p-ACC, ACC, and CPT1-b. E The levels of CPT1-b in H9c2, as analyzed by Immunofluorescence staining (n = 6, Scale bar = 100 μm). G The statistical analysis shows the positive expression of CPT1-b in H9c2. F, H TUNEL staining and positive cell Quantitative analysis (n = 6, Scale bar = 100 μm). I-J The expressions of Bcl-2, Bax, and Cleaved-caspase 3 were analyzed by immunoblotting in H9c2 in each group (n = 6). Data are represented as means ± SD, two-way ANOVA followed by Tukey’s post hoc test was used. *P < 0.05, **P < 0.01, and ***P < 0.001