Fig. 6

AICAR can reduce lipotoxicity mediated by C14. A ORO staining was used to quantify the lipid deposition (scale bar =50 μm, 100 μm). B Quantitative analysis of intracellular lipid droplets (n = 6). C H9c2 cells in each group were tested for triglyceride (n = 6). D Laser scanning confocal microscopy (LSCM) showed the non-oxidized lipid (shown as red) and oxidized lipid (shown as green), H9c2 cells in pretreated with either PA (0.3 mM), C14 (25 μM), or AICAR (1 mM) (Scale bar = 100 μm). E Statistical analysis of the fluorescence intensity of oxidized lipid expression from each group (n = 6). Data are represented as means ± SD, two-way ANOVA followed by Tukey’s post hoc test was used. *P < 0.05, **P < 0.01, and ***P < 0.001