Fig. 7

AICAR reduces lipid accumulation by alleviates the C14 mediated inhibition of the AMPK/ACC/CPT1 signaling pathway. A Western blotting was used to detect the expressions of P-AMPK, AMPK, P-ACC, ACC, and CPT1-b in each group (n = 6). B-D Quantification of p-AMPK, AMPK, p-ACC, ACC, and CPT1-b expressions. E The levels of CPT1-b in H9c2 were explored by immunofluorescence staining (n = 6, Scale bar = 100 μm). F The statistical analysis represents the positive expression of CPT1-b in H9c2. Data are represented as means ± SD, where two-way ANOVA followed by Tukey’s post hoc test was used. *P < 0.05, **P < 0.01, and ***P < 0.001