Fig. 8

AICAR reduces C14 mediated apoptosis. A A fluorescence microscope was used to explore the mitochondrial membrane potential and apoptosis (n = 6, Scale bar = 100 μm). B The statistical analysis represents levels of red and green fluorescence (n = 6). C The expressions of Bcl-2, Bax, and Cleaved-caspase 3 were analyzed by immunoblotting in H9c2 (n = 6). D Bcl-2, Bax, and Cleaved-caspase3 expression results are shown (n = 6). E The expressions of Bcl-2 were tested by Immunofluorescence staining (n = 6; Scale bar represents as 100 μm). F The statistical analysis represents the positive expression of Bcl-2 in H9c2. Data are represented as means ± SD, two-way ANOVA followed by Tukey’s post hoc test was used. *P < 0.05, **P < 0.01, and ***P < 0.001