Fig. 9

AICAR reduces C14 induced H9c2 cell fibrosis and hypertrophy. A The expressions of MYHC, MMP-9, TGF-β, and COL-1 were tested by immunoblotting in H9c2 (n = 6). B Quantification of MYHC, MMP-9, TGF-β, and COL-1 expressions (n = 6). C-D Laser scanning confocal microscopy (LSCM) images show the levels of MYHC and COL-1 in H9c2 (n = 6; Scale bar represents as 50 μm). E-F The statistical analysis represents the positive expression of MYHC and COL-1 in H9c2. Data are represented as means ± SD, two-way ANOVA followed by Tukey’s post hoc test was used. *P < 0.05, **P < 0.01, and ***P < 0.001