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Fig. 7 | Lipids in Health and Disease

Fig. 7

From: Palmitic acid causes increased dihydroceramide levels when desaturase expression is directly silenced or indirectly lowered by silencing AdipoR2

Fig. 7

Desaturase or SREBF silencing causes elevated dihydroceramide levels (data from BBA_2021_1 and BBA_2021_3). The sum of four sphingolipid species in HEK293 cells treated with the indicated siRNAs then cultivated in the presence of 200 μM PA are shown as box plots while their fatty acid composition is shown as heat maps. In the box plots, boxes indicate the 25th to 75th percentile while the whiskers indicate the data points still within 1.5 of the box range. For A-D and F-I, significant differences from the NT siRNA control were determined using one-way ANOVA and post-hoc Dunnett tests, with *P < 0.05, **P < 0.01 and ***P < 0.001. For the heat maps, the amount of each lipid species was normalized to the average of the NT + 200 μM PA treatment and the heat maps show fold differences from the mean across all treatments (each column is a replicate); only lipid species with significantly different levels among siRNAs in a given culture condition are included (ANOVA, q < 0.05)

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Lipids in Health and Disease

ISSN: 1476-511X