Fig. 1

Generation of adipocyte-specific PIP5K1c knockout mice. a qPCR analysis of PIP5K1c mRNA expression in different organs of 20-week-old WT and KO mice. b Body weight and c body weight gain of WT and KO mice fed with NCD for 20 weeks, N = 8–9/group. d Morphology of WT and KO mice fed with NCD. e Adipose tissue mass including eWAT, subWAT and BAT after sacrifice of mice, N = 8–9/group. f Organ weight of liver, heart and kidney after sacrifice of mice, N = 8–9/group. g Food intake and h water intake statistics of WT and KO mice fed with NCD. i Representative image of H&E staining of adipose tissue from WT and KO mice with NCD diet, Scale bar: 50 μm. (j, k) Glucose tolerance test j and Insulin tolerance test k in WT and KO mice fed an NCD, Right panel, area under the curve, N = 8–9/group. *P < 0.05; **P < 0.01