Fig. 4
From: Fat mass and obesity–associated protein promotes liver steatosis by targeting PPARα
Knockdown of FTO inhibits lipid accumulation in hepatocytes. A Western blotting analysis of the FTO protein level in HepG2 cells transfected with the FTO shRNA lentiviral (shFTO) or negative control (shNC) vector. B-E Oil Red O staining of HepG2 cells transfected with shFTO or shNC after treatment with palmitic acid (PA) (0.5 mM) or 2% bovine serum albumin (BSA) for 24 h (200x magnification). F Cellular contents of triglycerides (TG) in FTO knockdown and control HepG2 cells stimulated with 2% BSA and PA (0.5 mM) for 24 h (n = 3 independent experiments). *P < 0.05 vs. control group