Table 1 Primers used in real-time PCR
From: Fat mass and obesity–associated protein promotes liver steatosis by targeting PPARα
Gene name | Gene ID | Species | Primer Sequence (Forward: 5’-3’) | Primer Sequence (Reverse: 5’-3’) |
|---|---|---|---|---|
FTO | 79,068 | Homo sapiens | ACTTGGCTCCCTTATCTGACC | TGTGCAGTGTGAGAAAGGCTT |
PPARα | 5465 | Homo sapiens | TTCGCAATCCATCGGCGAG | CCACAGGATAAGTCACCGAGG |
GAPDH | 26,330 | Homo sapiens | TGGATTTGGACGCATTGGTC | TTTGCACTGGTACGTGTTGAT |
FTO | 26,383 | Mus musculus | TTCATGCTGGATGACCTCAATG | GCCAACTGACAGCGTTCTAAG |
GAPDH | 14,433 | Mus musculus | AGGTCGGTGTGAACGGATTTG | TGTAGACCATGTAGTTGAGGTCA |