Fig. 1

Measurement of high-density lipoprotein (HDL) function in patients with severe AS. HDL particles were isolated by PEG from women (F, red, A) and men (M, blue, B) with severe AS. ApoA-I containing particles were probed with human anti-apoA-I antibody and revealed by chemiluminescence. Molecular size markers are shown. (C) ApoA-I preβ1-HDL and α-HDL particles were quantified by Western blotting followed by densitometric scanning. (D) ABCA1-mediated cholesterol efflux capacity to HDL from women (red) and men (blue) with severe AS. (E) Formation of lipidated apoA-I particles from BHK cells expressing ABCA1 and separation by 2D-PAGGE. (F) LCAT activity (%/h) in plasma samples. After lipid extraction, ³[H]-unesterified cholesterol and ³[H]-cholesteryl ester were separated by thin liquid chromatography and assayed for radioactivity