Fig. 6

CAAT-CM effect on MDA-231 and SUM-149 cells motility. Representative photographs of (A) MDA-MB-231 and (B) SUM-149 BC cells in the wound healing assay at 0 hr and at 24 h. Scale bar, 200 μm. (C) The percentage of wound closure assay reveals a higher migratory potential of MDA-MB-231 cells compared with the control upon stimulation with 25% CAAT-CM. (D) The percentage of wound closure assay tended to show significance in SUM-149 cell migration compared with the control upon stimulation with 25% CAAT-CM. The area of wound closure was imaged at the indicated times and measured using the ImageJ software. Bar graphs represent a fold change ± SEM. ^**Represents P ≤ 0.01 as determined by the Student’s t-test