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Fig. 2 | Lipids in Health and Disease

Fig. 2

From: Hypoxia-induced MIF induces dysregulation of lipid metabolism in Hep2 laryngocarcinoma through the IL-6/JAK-STAT pathway

Fig. 2

MIF may be a key factor in hypoxia regulating lipid metabolism. A Venn diagram showed the intersection of hypoxia gene sets and fatty gene sets. B The mRNA expression of MIF, ENO2 and LDHA were verified by qRT-PCR. C The protein level of MIF was assessed by ELISA assay. Hep2 cells were treated with 25 µ M ISO-1. Then MIF protein level (C), TG (D) and NEFA (E) concentration were assessed. F NEFA concentration was assessed by NEFA assay. G QRT-PCR verified the mRNA expression of lipid metabolism-related genes. H CCK8 assay assessed the proliferation of Hep2 cells. I Representative images and statistical analysis of colony formation assay. The data are presented as mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.001

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Lipids in Health and Disease

ISSN: 1476-511X