Fig. 5
![Fig. 5](http://media.springernature.com/full/springer-static/image/art%3A10.1186%2Fs12944-022-01693-z/MediaObjects/12944_2022_1693_Fig5_HTML.png)
MIF antagonist ISO-1 inhibits the tumorigenicity of Hep2 cells in vivo. Four-week-old nude mice (five mice per group) were subcutaneously injected with the Hep2 cells (4 * 10.6 cells each mouse) and treated with ISO-1 at day eight. A The tumour growth curves in vivo. B Picture of isolated tumors and tumour weight. C Images and statistic results of a positive rate of Ki67. D Serum IL-6 level and (E) serum TG level were assessed by ELISA assay. F qRT-PCR was applied to detect relative mRNA expression of JAK/STAT gene sets. The data are presented as mean ± SD. *P < 0.05; ***P < 0.001