Fig. 5

PICRUSt recapitulates biological findings from the chronic-binge ApoH^−/− mouse model. A The functional profiling of microbial communities was predicted using the PICRUSt KEGG pathway. B-H Analysis of the sequencing data using STAMP software to predict potential metabolic differences. B Principal component analysis (PCA) plot using STAMP software. The x and y axes indicate principal components 1, 2, and 3 accounting for 85.2%, 9.0%, and 2.8% of the total variation in predicted functions, respectively. C, D Distribution of relative abundance of “metabolism” and “lipid metabolism” between different groups (P < 0.05). E Heatmap illustrating the abundance of selected active KEGG pathways in WT mice given alcohol and the pair-fed control diet. F Carbohydrate metabolism shown as the active KEGG pathways in WT and ApoH^−/− mice with alcohol feeding (P = 0.032). G, H Metabolism and lipid metabolism pathways were activated and differed significantly between ApoH.^−/− mice fed alcohol and control diets (P = 0.017 and P = 0.030, respectively)