Fig. 1
From: FGF15 promotes hepatic NPC1L1 degradation in lithogenic diet-fed mice
Hepatic NPC1L1 had no effect on bile cholesterol reabsorption in LD-fed mice. The WT and mNPC1L1hepatic-OE mice were fed with 8-week chow diet, HFCD and LD with or without EZE treatment prior to the indicated experiments. A Immunoblotting analysis was applied to determine the hepatic expressions of GFP and NPC1L1 in chow diet-fed WT, AAV-GFP, and AAV-mNPC1L1 mice, with tubulin used as loading controls. The band densities of western blot images were analyzed with the ImageJ software and indicated below the bands, normalized to their loading controls. B Biliary cholesterol monohydrate crystals aggregating into gallstones and true gallstones examined by polarizing light microscopy. Original magnification, × 40. C Biliary cholesterol (a), bile acids (b), phospholipid (c) and (d) CSIs in a-d panels. D Cholesterol (a) and triglyceride (b) from liver tissues. E Cholesterol (a) and triglyceride (b) from plasma. Mann-Whitney U-test for data with non-normal distribution and Student’s t-test for normal distributions. *P < 0.05, **P < 0.01, ns no significance. WT, wide-type; EZE, ezetimibe; HFCD, high fat-cholesterol diet; LD, lithogenic diet; CSI, cholesterol saturation index