Fig. 3
From: FGF15 promotes hepatic NPC1L1 degradation in lithogenic diet-fed mice
Hepatic NPC1L1 protein degradation in LD-fed mice. The mNPC1L1hepatic-OE mice were fed with 2-week or 8-week chow diet, HFCD and LD prior to the indicated experiments. Immunoblotting analysis was applied to assess the expression of NPC1L1 in intestine and liver (A), and GFP in liver (B), with tubulin controls. C upper panel: Immunoblot of NPC1L1 and ubiquitin (Ub) in the liver collected from LD-fed mice. The antibody against tubulin served as the internal control. Lower panel: NPC1L1 ubiquitination was assessed by immunoprecipitating NPC1L1 and immunoblotting using anti-Ub antibody. The band densities of western blot images were analyzed with the ImageJ software and indicated below the bands, normalized to their loading controls. Student’s t-test. n = 3. HFCD, high fat-cholesterol diet; LD, lithogenic diet; Ub, ubiquitination