Table 1 The primers of RT-PCR
From: New insight into the role of lipid metabolism-related proteins in rheumatic heart valve disease
Accession No. | Gene | Primer sequence (5′ to 3′) | Annealing temperature (°C) | |
|---|---|---|---|---|
NM_002046 | H-GAPDH-S | GGAAGCTTGTCATCAATGGAAATC | 168 | 60 |
H-GAPDH-A | TGATGACCCTTTTGGCTCCC | 60 | ||
NM_001251.2 | H-CD68(rz)-S | CCAACAAAACCAAGGTCCAGG | 99 | 60 |
H-CD68(rz)-A | GGTCCTGCATGAATCCAAAGC | 60 | ||
NM_000019.4 | H-ACAT1-S | AAGTAAAGCAGCATGGGAAGC | 267 | 60 |
H-ACAT1-A | GCCAGTGGTGTAACATTGAGCC | 60 | ||
XM_011518342.3 | H-ABCA1-S | ATGGCTGATGCCCTGGAAAG | 117 | 60 |
H-ABCA1-A | AATGAGGAAGAACACCACCCC | 60 | ||
NM_000594.3 | H-TNF-α-S | TCTACTCCCAGGTCCTCTTCAAG | 201 | 60 |
H-TNF-α-A | GGAAGACCCCTCCCAGATAGA | 60 | ||
NM_000572.3 | H-Il-10(RZ)-S | ACTGCTCTGTTGCCTGGTCCT | 232 | 60 |
H-Il-10(RZ)-A | GGCTTGGCAACCCAGGTAA | 60 | ||
NM_002982.3 | H-MCP-1-S | CTCGCTCAGCCAGATGCAAT | 113 | 60 |
H-MCP-1-A | CACTTGCTGCTGGTGATTCTTCT | 60 |