Fig. 11

Morphology and identification of exosomes. A Serum exosomes from different groups were observed by transmission electron microscopy (TEM) at a scale of 100 nm and are indicated with arrowheads. B Particle size analysis showed that the particle size of each exosome ranged from 40 nm to 100 nm. C The expression levels of TSG101, CD9 and CD63 were determined by western blot. These proteins were extracted from exosomes isolated from rat serum and were always used as the labeled proteins in exosomes