Fig. 7
From: Dietary fat induced chylomicron-mediated LPS translocation in a bicameral Caco-2cell model
Effect of digested palm oil and sunflower oil on paracellular and transcellular translocation by Caco-2 cells. Effect of digested palm oil and sun flower oil on translocation of FD4 (A) and LPS (B) to the basolateral side of Caco-2 cells. Digest control, digested palm oil and digested sunflower oil were diluted 1:50 in DMEM w/o phenol red and w/o FBS. Caco-2 cells were coincubated with digested oils (1:50), 0 and 750 ng/mL LPS and 250 µg/ml FD4 for 24 h. Basolateral FD4 was calculated using a calibration curve and compared to the control digest. LPS was measured using the Pyrochrome® assay. These exposures were conducted as a single-cell experiment