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Fig. 3 | Lipids in Health and Disease

Fig. 3

From: Exosomal IL-8 derived from Lung Cancer and Colon Cancer cells induced adipocyte atrophy via NF-κB signaling pathway

Fig. 3

LLC and C26-derived EVs conferred lipolysis of 3 T3-L1 adipocytes. A-B Western blot and the densitometric analysis of UCP1, PGC1α and p-p65, total p65 protein expression in total extracts and nuclear extracts of 3 T3-L1 adipocytes, co-cultured with LLC versus C26-conditioned medium and EVs-free conditioned medium. C-D Western blot of PGC-1α and UCP1 in 3 T3-L1 adipocytes treated with LLC-EVs versus C26-EVs at various doses and the corresponding densitometric analyses (n = 3). E Oil red O staining of 3 T3-L1 adipocytes co-cultured with LCM, CCM, LCM-dep, CCM-dep, LLC-EVs (10 μg), C26-EVs (10 μg), IL-6 (50 ng/ml) and TNF-α (50 ng/ml). Bar = 100 μm. *P < 0.05, **P < 0.01,***P < 0.001

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Lipids in Health and Disease

ISSN: 1476-511X