Fig. 2

Role of ATG2B in NASH telephone model. A Relative mRNA stages of ATG2B as decided by using Western Blot and qRT-PCR. Error bars represent the SD. B HepG2 cells had been transfected with pcDNA3.1-NC or ATG2B and then dealt with or besides FFA (1 mM) for forty-eight hours. Representative pics exhibit the handled HepG2 cells (scale bar: four hundred μm) and Oil Red O staining. C TG ranges in traditional supernatants. D ALT and AST range in way of life supernatants. E H₂O₂ concentration, ROS level, and ATP attention in HepG2 cells. F The expressions of IL-6, TNF-α, and IL-10 were detected by ELISA. *P < 0.05, **P < 0.01, ***P < 0.001