Fig. 1

Atherosclerotic lesions and abnormal blood lipid levels of ApoE^−/− mice fed a high-cholesterol diet. A-B Representative images of oil red O staining of the aortic arch and quantitative analysis of the percentage of oil red O positive staining area. Original magnification: 40 × . C Representative transmission electron microscope images of subcutaneous lipid droplets in the aortic arch. Original magnification: 1.2 k × . D The four levels of blood lipids (left) and the ratio of low-density lipoprotein to high-density lipoprotein (right) by kits. E Lipids in plasma samples were extracted and detected by HPLC-Q-TOF/MS. F Volcano diagrams of the ApoE^−/− + NF group and the ApoE^−/− + HF group. G PCA score chart, showing the difference between the C57BL/6 + NF group (green), the ApoE^−/− + NF group (blue), and the ApoE^−/− + HF group (red) in plasma samples. H PLS-DA score chart, showing the difference between the C57BL/6 + NF group (green), the ApoE^−/− + NF group (blue), and the ApoE^−/− + HF group (red) in plasma samples. I The heat map showed the difference in plasma lipid levels in each group. J-M The box plot reflected the relative content of each type of lipid. In all experiments, n = 6, the P value indicates the comparison with the ApoE^−/− + NF group. Values are expressed as mean ± SEM