Fig. 4

The cholesterol metabolism of intestinal Caco-2 cells is mediated by LXRα. A-B Representative IF images and quantitative analysis of ZO-1 in Caco-2cells. C-D Representative images of oil red O staining of Caco-2 cells and quantitative analysis of the percentage of oil red O positive staining area. E–H Lipid levels in Caco-2 cells tested by kits. I The mRNA expression of targets related to lipid metabolism in Caco-2 cells. J-K Representative IF images and quantitative analysis of NPC1L1, ABCG5, ABCG8, LXRα in Caco-2cells. L-P representative images of oil red O staining of Caco-2 cells incubated with 100 μmol of cholesterol for 24 h and quantitative analysis of the positive area; Q-R Representative Western blot and relative quantitative analysis of NPC1L1, ABCA1, ABCG5, ABCG8, and LXRα after incubating Caco-2 cells with 100 μmol of cholesterol for 24 h. In the above experiments, n = 5, the P value indicates the comparison with the control group. Values are expressed as mean ± SEM