Fig. 6
From: HDL and LDL have distinct, opposing effects on LPS-induced brain inflammation
Peripherally administered LPS in combination with HDL has distinct effects on the brain metabolome and significantly increases the level of brain endotoxin. Adult male C57BL/6 mice received an intraperitoneal injection of either saline (n = 9), LPS (0.5 mg/kg, n = 11) or LPS mixed with lipoprotein immediately prior to administration (HDL, n = 6, 20 mg/kg; LDL, n = 5, 20 mg/kg). Metabolites driving differences between treatment groups by principal component analysis were evaluated. Relative amounts of lactate (Ai), adenosine monophosphate (Aii), aspartate (Aiii), valine (Aiv), NAD (Av) and citrate (Avi) were determined. Brain lysates were analysed for the presence of endotoxin using a limulus amoebocyte lysate assay (B). Data are presented as Tukey boxplots analysed by one-way ANOVA with Sidak’s test for multiple comparisons, *p < 0.05, **p < 0.01, ****p < 0.0001