Fig. 8

Cholesterol efflux and antioxidative capacities of each HDL subclass in human and rat samples. a Cholesterol efflux capacity of the S/M/L-HDL subclasses in human and rat samples. HDL subclasses were separated by FPLC and prepared for cholesterol efflux examination. J774A.1 cells were preloaded with ³H-cholesterol (1 Ci/ml) for 24 h. ³H-cholesterol efflux was detected after incubation with a equal volume of HDL subclass for 4 h. b Antioxidative capacity of the S/M/L-HDL subclasses in human and rat samples. HDL antioxidative ability was reflected as the antioxidative index, which was calculated as the endpoint fluorescence of DHR incubated with ox-LDL corrected for the optical density and divided by the optical density of DHR coincubated with ox-LDL and a equal volume of HDL subclass. *, P < 0.05