Fig. 4

A-C BiP expression in oxLDL treated cells at various time points. Western blots depicting GRP78/BiP expression in HepG2 cells overexpressing empty vector, WT/variants of LDLR treated with 100 µg per ml of oxLDL at (A) 16 h, (B) 24 h, and (C) 48 h. Non- transduced HepG2 cells were used as controls to monitor the expression pattern of endogenous BiP expression at various time points. Non-treated or oxLDL-treated conditions for each cell type evaluated are marked by ‘-’ and ‘ + ’. Data are represented as mean + SD. 2-way ANOVA using Turkey’s post hoc test was used to compare BiP expression in non-treated (-) or treated ( +) conditions of each cell type with the non-treated and non-transduced HepG2 control cells (HepG2⁻). P < 0.05 = *, P < 0.01 = **, P < 0.001 = ***, P < 0.0001 = **** is represented for the comparisons between non-transduced HepG2 control cells (HepG2⁻) versus non-treated or treated HepG2^WT−LDLR, HepG2^D482H−LDLR, and HepG2^C667F−LDLR. P < 0.05 = # when the treated cells were compared with the respective non-treated cell types. ns = not significant, n = 3 replicates