Fig. 5
From: Heme oxygenase 1 alleviates nonalcoholic steatohepatitis by suppressing hepatic ferroptosis
HO-1 inhibited hepatic ferroptosis in steatotic hepatocytes. (A) Ferrous iron staining (red) in AML12 cells. Nuclei were stained using Hoechst 33,342 (blue) (scale bar = 100 μm). (B, C, D, E) The total iron and ferrous iron levels in AML12 and HepG2 cells. (F, G) Western blot analysis of SLC7A11, GPX4, Ferritin, ACSL4, LOX in AML12 and HepG2 cells. Values are the mean ± SD. *P < 0.05, **P < 0.01 compared with the control group; #P < 0.05, ##P < 0.01 compared with the FFA group