Table 1 The gradient elution of lipids and BAs detection
| Â | Time (min) | Pump B concentration |
|---|---|---|
Lipids detection | 0–2 | 20–30% |
2–4 | 30–60% | |
4–9 | 60–85% | |
9–14 | 85–90% | |
14–15.5 | 90–95% | |
15.5–17.3 | 95%-20% | |
17.3–20 | 20% | |
BAs detection | 0–0.5 | 5%-40% |
0.5–4.5 | 40–50% | |
4.5–7.5 | 50–75% | |
7.5–10 | 75%-95% | |
10–12 | 95%-5% |