Fig. 6

Overexpression of Sirt6 results in enhanced plaque stability. A Immunohistochemical analysis of macrophages (CD68) and α-smooth muscle actin (SMA); Collagen was stained using Masson’s reagent, and the lipid vacuole was stained with Oil red O. B–C Examining CD68 positive area and the plaque stabilisation score quantitatively. Data are presented as mean ± SE (One way ANOVA showed that there was statistical significance among groups, ^*P < 0.05 vs ApoE^−/− mice [n = 10]; ^#P < 0.05 vs ApoE^−/−: Sirt6^−/− mice [n = 10]). D Plaque slices were immunostained with a fluorescent anti-MMP2 antibody. E A quantitative study of the plaque area that showed positive staining for MMP2; data are presented as mean ± SE (One way ANOVA showed that there was statistical significance among groups, ^*P < 0.05 vs ApoE^−/− mice [n = 10]; ^#P < 0.05 vs ApoE^−/−: Sirt6Tg [n = 10])