Fig. 4

Anlotinib mediates key genes and enzymes required for cholesterol synthesis and uptake in human lung cancer cells. A A549 and H460 cells were treated with various concentrations of anlotinib. LDLR, SREBP1, SREBP2, HMGCR, AMPK, mTOR, ACC, SCD1 and FASN mRNA expression levels were determined. B LDLR, SREBP1, nuclear SREBP1 (nSREBP1), SREBP2, nuclear SREBP2 (nSREBP2) and HMGCR protein expression levels along with their respective quantitative histograms. *P < 0.05; **P < 0.01 by Student’s t test