Fig. 2From: Periostin drives extracellular matrix degradation, stemness, and chemoresistance by activating the MAPK/ERK signaling pathway in triple–negative breast cancer cellsACM and PA induce the expression and secretion of periostin in TNBCs. a–b. MDA–MB–231 and SUM159PT cells were treated with NC or ACM for 24 h, and then the POSTN mRNA and protein levels were determined by qPCR (a) and Western blotting (b) analysis. c–d. MDA–MB–231 and SUM159PT cells were treated with vehicle or PA for 24 h, and then the POSTN mRNA and protein levels were determined by qPCR (c) and Western blotting (d) analysis. e–f. The concentration of periostin in the cell culture medium was determined by ELISA. NC: negative control; ACM blank: ACM alone; ACM with: ACM cocultured with TNBCs. ns: no significance; *P < 0.05Back to article page