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Fig. 6 | Lipids in Health and Disease

Fig. 6

From: Periostin drives extracellular matrix degradation, stemness, and chemoresistance by activating the MAPK/ERK signaling pathway in triple–negative breast cancer cells

Fig. 6

Periostin activates the MAPK signaling pathway to promote extracellular matrix degradation, stemness, and chemoresistance in TNBCs. (a) Four samples representative of the transcriptome heatmap. (b) Volcano plot of the RNA–seq results. c–d. GO term enrichment analysis in the BP category (c) and KEGG pathway enrichment analysis (d) of the upregulated genes in the rhPeriostin group compared with the IgG group. The bars marked in red were the ones we focused on. e–f. Western blotting analysis of the indicated protein levels in MDA–MB–231 cells treated with IgG or rhPeriostin and/or U0126–EtOH for 24 h. The densitometry results of p–ERK1/2 (upper and bottom strips) were expressed as fold change in the protein levels when compared with IgG–treated MDA–MB–231 cells after being normalized to β–actin (e). g–h. qPCR analysis of the indicated gene expression in MDA–MB–231 cells treated with IgG or rhperiostin and/or U0126–EtOH for 24 h. i. CCK–8 analysis of the cellular viability when MDA–MB–231 cells were treated with 3 µg/ml PTX in the indicated conditions. *P < 0.05

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Lipids in Health and Disease

ISSN: 1476-511X