Fig. 1
From: Suramin, an antiparasitic drug, stimulates adipocyte differentiation and promotes adipogenesis
Effects of suramin on the toxicity of fascial preadipocytes. Fascial preadipocytes were added to suramin at 35, 70, 105, and 140 µM. (A) The CCK-8 assay was used to measure the absorbance at 450 nm to reflect cell viability for 4 days. (B) LDH released from fascial cells treated for 5 days with different concentrations (35–140 µM) of suramin. LDH activity in the cell lysate and culture medium was measured at an absorbance of 440 nm via a microplate reader. LDH activity release rate was calculated as follows: (supernatant LDH activity)/(supernatant LDH activity + lysate LDH activity). Data were obtained from three independent experiments and are shown as mean ± STD. Statistical analysis was performed using one-way or two-way ANOVA. *P < 0.05, **P < 0.01, ***P < 0.001