Table 2 Linear range, regression coefficient, and calibration equation of LCFAs
LCFA | Range (μM) | R2 | ISO-range (μM) | ISO-R2 | Rf value | RSD of Rf (%) | LOD (nM) | LOQ (nM) |
|---|---|---|---|---|---|---|---|---|
12:0 | 0.05-40.01 | 0.9996 | 0.05-40.41 | 0.9997 | 1.33 | 9.82 | 5.01 | 25.01 |
14:0 | 0.51-199.98 | 0.9993 | 0.51-201.78 | 0.9996 | 1.18 | 15.29 | 1.02 | 5.01 |
16:0 | 25.01-14998.59 | 0.9990 | 25.19-15115.58 | 0.9993 | 0.58 | 9.01 | 1.01 | 5.01 |
16:1 | 1.01-199.99 | 0.9994 | 1.06-212.57 | 0.9994 | 1.21 | 1.21 | 49.99 | 100.01 |
18:0 | 10.01-4000.32 | 0.9993 | 10.07-4028.44 | 0.9994 | 1.20 | 2.27 | 1.01 | 5.02 |
18:1 | 25.01-7498.37 | 0.9991 | 25.18-7555.01 | 0.9991 | 0.89 | 2.88 | 1.01 | 5.01 |
18:2 | 25.01-3000.23 | 0.9993 | 25.09-3010.92 | 0.9997 | 1.31 | 7.33 | 1.01 | 5.01 |
20:0 | 0.05-50.01 | 0.9997 | 0.05-50.33 | 0.9999 | 2.02 | 11.73 | 5.02 | 49.98 |
20:4 | 0.51-400.04 | 0.9990 | 0.51-410.55 | 0.9994 | 10.42 | 11.14 | 5.01 | 50.02 |
20:5 | 10.01-499.92 | 0.9996 | 10.16-508.18 | 0.9992 | 1.08 | 15.14 | 4999.82 | 9999.97 |
22:0 | 0.05-50.01 | 0.9998 | 0.05-50.59 | 0.9997 | 1.42 | 7.57 | 25.01 | 33.33 |
22:6 | 3.25-499.87 | 0.9993 | 3.56-533.35 | 0.9993 | 3.16 | 8.34 | 1000.01 | 2499.99 |
24:0 | 0.51-80.01 | 0.9991 | 0.05-80.87 | 0.9993 | 0.93 | 1.01 | 5.01 | 500.01 |
26:0 | 0.25-80.01 | 0.9990 | 0.25-80.82 | 0.9993 | 3.66 | 17.56 | 25.01 | 49.99 |