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Fig. 3 | Lipids in Health and Disease

Fig. 3

From: A regulatory element associated to NAFLD in the promoter of DIO1 controls LDL-C, HDL-C and triglycerides in hepatic cells

Fig. 3

Quantification of neutral lipids and biochemical assays of UpsDIO1 CRISPR-Cas9 HepG2 mutants. A Representative pictures of merged channels for Hoechst and Bodipy dyes of HepG2 cells treated with 120 µM oleic acid (OA). Pictures were taken at 10X objective magnification. Bodipy quantification of UpsDIO1 mutants (gRNA-1 and gRNA-2) show differential accumulation of neutral lipids at 40 and 120 µM OA in comparison to wildtype (WT) HepG2. In total 9 pictures were taken from each culture (n = 3) and the mean and standard deviation of the mean (SEM) per cell is presented. B Biochemical quantification of culture cell extracts after treatment with 120 µM OA for triglycerides, total cholesterol, LDL-C and HDL-C. For Intracellular lipid accumulation and biochemical quantification P-value derived from unpaired T-Test. *P =  < 0.05,**P =  < 0.01, ***P =  < 0.001

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Lipids in Health and Disease

ISSN: 1476-511X