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Fig. 7 | Lipids in Health and Disease

Fig. 7

From: Topical rhubarb charcoal-crosslinked chitosan/silk fibroin sponge scaffold for the repair of diabetic ulcers improves hepatic lipid deposition in db/db mice via the AMPK signalling pathway

Fig. 7

Predicting the AMPK Signaling Pathway Regulation through Network Pharmacology and Validating with Molecular Experiments. A Venn diagram of rhubarb and NAFLD-related targets. B KEGG pathway enrichment analysis. C Relative mRNA expression of Prkaa1, Srebf1, Fasn and Acaca. D Protein expressions of p-AMPK, t-AMPK, SREBP1 precursor (pSREBP1), mature SREBP1 (mSREBP1), FASN, and ACC in mice by Western blotting. E Quantitative analysis of the relative protein expression of p-AMPK, pSREBP1, mSREBP1, FASN, and ACC in liver. Stain-free technology was used as loading control. Uncropped stain-free gels of all samples used for total protein normalization are shown in Fig S3. The values reported in the figure represent the means ± SEM (n = 6). ###P < 0.001, ##P < 0.01, #P < 0.05 compared with the db/m group; ***P < 0.001, **P < 0.01, *P < 0.05 compared with the RC group

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Lipids in Health and Disease

ISSN: 1476-511X