Fig. 6

Influence of NOB on RAW264.7 cells viability. RAW264.7 cells were intervened by OxLDL (50 µg/mL) for 48 h to construct an ex vivo model of atherosclerosis. (A) The direct effect of different concentrations of NOB on RAW264.7 cell viability was analyzed by CCK-8 method (n = 6). (B) On the basis of an ex vivo model of atherosclerosis, CCK-8 was used to detect the ameliorative effect of NOB on cell viability (n = 6). The effect of OxLDL on macrophage migratory capacity (at six random locations; 200× magnification) and the ameliorative effect of NOB were observed via a scratch assay (C, D) (n = 6). The ameliorative effect of NOB (50 µM) on OxLDL-induced apoptosis was examined using flow cytometry (E, F) (n = 3). *P < 0.05, **P < 0.01, ****P < 0.0001 vs. the control group; #P < 0.05, ##P < 0.01, ####P < 0.0001 vs. the OxLDL group