Skip to main content
Fig. 1 | Lipids in Health and Disease

Fig. 1

From: PDGFRβ + cell HIF2α is dispensable for white adipose tissue metabolic remodeling and hepatic lipid accumulation in obese mice

Fig. 1

HIF2α inactivation does not impact adipogenic differentiation or inflammatory response in adipose tissue PDGFRβ + cells. A Schematic illustration of Epas1-bKO mice. Pdgfrb-CreERT2; Epas1loxP/loxP (Epas1-bKO) mice are generated by cross-breeding animals carrying floxed Espa1 (HIF2α protein encoding gene) alleles (Epas1loxP/loxP) to Pdgfrb-CreERT2 transgenic mice. Pdgfrb-CreERT2 littermates were used as control animals. B mRNA levels of Epas1 in isolated gWAT PDGFRβ + cells from TAM-treated control and Epas1-bKO mice. n = 3 per group. C Western blot analysis of HIF2α and β-ACTIN proteins in isolated gWAT PDGFRβ + cells from TAM-treated control and Epas1-bKO mice after the indicated treatments. D Representative Oil Red O-staining photos of isolated gWAT PDGFRβ + cells after the induction of in vitro adipogenesis with the indicated treatments. E mRNA levels of the indicated mature adipocyte genes in isolated gWAT PDGFRβ + cells after the induction of in vitro adipogenesis with the indicated treatments. n = 4 per group

Back to article page

Lipids in Health and Disease

ISSN: 1476-511X