Fig. 3

No apparent systemic effects caused by PDGFRβ + cell HIF2α inactivation in diet-induced obesity. A Monitoring of weekly body weights in control and Epas1-bKO mice during 8 weeks of HFD feeding. n = 6 per group. B Body composition (% of fat mass and lean mass) of control and Epas1-bKO mice after HFD feeding. n = 6 per group. C Glucose tolerance test (GTT) of HFD-fed control and Epas1-bKO mice. n = 6 per group. D Insulin tolerance test of (ITT) of HFD-fed control and -bKO mice. n = 6 per group. E Western blot analysis of phosphorylated AKT (pAKT), total AKT and β-actin in liver extracts from control and Epas1-bKO mice after HFD feeding. For quantification, intensity of pAKT band is normalized to that of total AKT band, and intensity of total AKT is normalized to that of β-actin. F Western blot analysis of phosphorylated AKT (pAKT), total AKT and β-actin in soleus muscle extracts from control and Epas1-bKO mice after 8 weeks of HFD feeding. For quantification, intensity of pAKT band is normalized to that of total AKT band, and intensity of total AKT is normalized to that of β-actin. G Hepatic TG and CHO contents of control and Epas1-bKO mice after 8 weeks of HFD feeding. n = 6 per group. H Representative liver H&E staining images of HFD-fed control and Epas1-bKO mice. Scale bar denotes 200 µM