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Fig. 1 | Lipids in Health and Disease

Fig. 1

From: Circulating ceramide levels and ratios in Emirati youth under 18 years: associations with cardiometabolic risk factors

Fig. 1

Integrated chromatograms, stability, and specificity of the liquid chromatography-mass spectrometry (LC-MS/MS) ceramide quantification assay. During the 5-minute run, Mobile Phase A comprised 10 mM ammonium formate and 0.1% formic acid in LC-MS grade water. Mobile Phase B contained 10 mM ammonium formate and 0.1% formic acid in LC-MS grade water/acetonitrile/isopropanol (5%: 47.5%: 47.5%) (A). Ceramide metabolites and internal standards were separated adequately based on molecular weights and polarities (B-C). The stability of ceramide metabolites over three freeze/thaw cycles showed percentage changes represented by blue, orange, and grey bars after 24, 48, and 72 h, respectively. Quality control levels confirmed percentage changes within ± 7% for all metabolites CerC16:0 (D), CerC18:0 (E), CerC22:0 (F), CerC24:0 (G), and CerC24:1 (H). Overlaid chromatograms of blank samples (I) confirmed method specificity, showing no interfering peaks at the respective retention times for ceramide metabolites. IS, Internal Standard; %change, stability

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Lipids in Health and Disease

ISSN: 1476-511X