Circulating ceramide levels and ratios in Emirati youth under 18 years: associations with cardiometabolic risk factors

Table 2 The Multiple Reaction Monitoring (MRM) parameters of ceramide metabolites and corresponding internal standards

No.	Ceramide (Cer)	Mass (g/mol)	Precursor (m/z)	*Product (m/z)	Collision energy (eV)
1	CerC16:0	537.901	520.59	264.33 282.34 252.31	20 20 24
2	CerC18:0	565.95	548.57	264.33 282.34 252.31	22 24 26
3	CerC22:0	622.06	604.62	264.33 282.34 252.31	24 26 30
4	CerC24:0	650.11	632.67	264.33 282.34 252.31	34 30 30
5	CerC24:1	648.09	630.655	264.33 282.34 252.31	28 28 30
6	CerC16:0-d7	544.94	527.51	271.3 289.36 259.28	22 22 26
7	CerC18:0-d7	572.99	555.62	271.29 289.36 259.34	22 24 24
8	CerC24:0-d7	657.156	639.72	271.29 289.35 259.33	30 30 32
9	CerC24:1-d7	655.14	637.45	271.35 289.35 259.33	28 26 26

*The ceramide generate the optimal transitions of [M + H-18] ⁺ to 264 m/z (mass-to-charge ratio)
CerC16:0 [C16:0-ceramide (d18:1/16:0)]; CerC18:0 [C18:0-ceramide (d18:1/18:0)]; CerC22:0 [C22:0-ceramide (d18:1/22:0)]; CerC24:0 [C24:0-ceramide (d18:1/24:0)]; CerC24:1 [C24:1-ceramide (d18:1/24:1(15Z))]; CerC16:0-d7 [C16:0-ceramide-d7 (d18:1-d7/16:0)]; CerC18:0-d7 [C18:0-ceramide-d7 (d18:1-d7/18:0)]; CerC24:0-d7 [C24:0-ceramide-d7 (d18:1-d7/24:0)]; CerC24:1-d7 [C24:1-ceramide-d7 (d18:1-d7/24:1(15Z))

ISSN: 1476-511X