Circulating ceramide levels and ratios in Emirati youth under 18 years: associations with cardiometabolic risk factors

Table 3 The liquid chromatography-mass spectrometry (LC-MS/MS) ceramide quantification assay validation results

N	Ceramide	*QC samples	Concentration (µM)	Inter-day			Intra-day			Recovery (%)	LOD (µM)	R²	Linear Range (µM)
N	Ceramide	*QC samples	Concentration (µM)	Precision (CV%)	Accuracy (PE%)	SD	Precision (CV%)	Accuracy (PE%)	SD	Recovery (%)	LOD (µM)	R²	Linear Range (µM)
1	CerC16:0	QCH	0.27	3.09	87.93	4.07	1.90	87.27	2.49	64.82	0.00016	0.997	0.0036–0.46
		QCM	0.07	4.08	98.89	1.51	4.78	97.23	1.74	47.46
		QCL	0.02	2.01	100.42	0.19	0.98	101.14	0.09	51.48
		LLOQ	0.0036	4.21	111.41	0.09	2.52	114.70	0.06	68.62
2	CerC18:0	QCH	0.16	3.97	89.79	3.21	2.35	88.38	1.87	66.37	0.00034	0.996	0.0021-0.27
		QCM	0.04	5.23	100.03	1.18	6.87	100.19	1.55	44.12
		QCL	0.0099	2.98	99.50	0.17	4.12	99.29	0.23	39.97
		LLOQ	0.0021	2.68	100.85	0.03	2.46	102.28	0.03	59.31
3	CerC22:0	QCH	0.96	2.75	88.72	14.63	2.97	89.08	15.87	66.61	0.00045	0.998	0.012–1.54
		QCM	0.23	4.05	93.89	5.70	4.58	95.30	6.55	44.12
		QCL	0.06	3.64	98.89	1.35	3.48	96.74	1.26	35.20
		LLOQ	0.012	2.32	100.73	0.18	1.60	100.43	0.13	52.62
4	CerC24:0	QCH	0.96	3.28	88.64	17.42	3.59	88.79	19.10	66.05	0.00045	0.998	0.012–1.54
		QCM	0.23	4.37	94.15	6.16	5.15	95.21	7.36	43.53
		QCL	0.06	4.52	99.52	1.69	3.84	96.69	1.39	36.59
		LLOQ	0.012	3.97	106.53	0.33	2.19	103.37	0.18	54.44
5	CerC24:1	QCH	0.96	1.15	87.23	6.04	0.86	86.78	4.48	60.37	0.00045	0.998	0.012–1.54
		QCM	0.23	5.07	95.09	7.23	5.91	94.54	8.38	40.01
		QCL	0.06	3.11	93.52	1.09	3.74	92.57	1.30	43.51
		LLOQ	0.012	2.14	101.42	0.17	2.04	101.71	0.16	52.75

*Ceramide (Cer) quality control samples (QC) at four levels (QCH [Quality Control High]; QCM [Quality Control Medium]; QCL [Quality Control Low]; LLOQ [ Lower Limit of Quantification])
CerC16:0 [C16:0-ceramide (d18:1/16:0)]; CerC18:0 [C18:0-ceramide (d18:1/18:0)]; CerC22:0 [C22:0-ceramide (d18:1/22:0)]; CerC24:0 [C24:0-ceramide (d18:1/24:0)]; CerC24:1 [C24:1-ceramide (d18:1/24:1(15Z))]; LOD [Limit of Detection]; SD [Standard Deviation]; CV% [percent coefficient of variation]; PE% [percent error]; R² [square of the correlation coefficient]

ISSN: 1476-511X