Table 4 Comparative results of the liquid chromatography-mass spectrometry (LC-MS/MS) ceramide quantification assay in this study and other relevant studies
Reference | Ceramide | LOD (µM) | LLOQ (µM) | Inter-day Precision (%CV) | Intra-day Precision (%CV) | Inter-day Accuracy (%PE) | Intra-day Accuracy (%PE) | Extraction Method | % Recovery | Injection Volume (µl) | R2 | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
Type | Extraction Solvent | |||||||||||
This Study | CerC16:0 | 0.00016 | 0.0036 | 2.0–4.2 | 0.9–4.7 | 87.9–111.4 | 87.2–114.7 | LLE | C6H14/ EtAc | 47.4–68.6 | 2 | 0.997 |
CerC18:0 | 0.00034 | 0.0021 | 2.6–5.2 | 2.3–6.8 | 89.7–100.8 | 88.3–102.2 | 39.9–66.3 | 0.996 | ||||
CerC22:0 | 0.00045 | 0.012 | 2.3–4.0 | 1.6–4.5 | 88.7–100.7 | 89.1–100.4 | 35.2–66.6 | 0.998 | ||||
CerC24:0 | 0.00045 | 0.012 | 3.2–4.5 | 2.1–5.1 | 88.6–106.5 | 88.8–103.4 | 36.5–66.0 | 0.998 | ||||
CerC24:1 | 0.00045 | 0.012 | 1.1–5.0 | 0.8–5.9 | 87.2–101.4 | 86.8–101.7 | 40.0–60.3 | 0.998 | ||||
Jiang et al. (25) | CerC22:0 | – | 0.032 | 1.8–3.2 | 1.4–3.4 | 91.1–99.8 | 90.2–100 | PPT | IPA/CHCl3 | 109 | 5 | 0.999 |
CerC24:0 | – | 0.12 | 1.2–3.6 | 0.5–3.4 | 93.2–99.6 | 92.5–99.8 | 114 | 0.997 | ||||
Kauhanen et al. (24) | CerC16:0 | – | 0.08 | 7–8.9 | 5.9–6.8 | 97.8–99.8 | 97–99.5 | PPT | IPA/EtAc | 99.9–102.1 | 5 | 0.992 |
CerC18:0 | – | 0.03 | 6.5–10.9 | 4.2–8.2 | 90.1–95.8 | 85.1–90.7 | 100.9 − 106.6 | 0.993 | ||||
CerC24:0 | – | 0.86 | 6.6 − 11.8 | 5.0–6.3 | 91.4–96.5 | 86.9–90.5 | 98.8–99.5 | 0.998 | ||||
CerC24:1 | – | 0.29 | 5.7–10 | 3.7–6.8 | 94.8–97.5 | 89.4–92.9 | 102.1 − 109.1 | 0.996 | ||||
Huang et al. (16) | CerC16:0 | – | 0.005 | 1.5–6.2 | 2.4–6.1 | 96.4–99.7 | 94.7–99.1 | PPT | MeOH | 102.3 − 104.5 | 10 | 0.999 |
CerC18:0 | – | 0.00099 | 2.8–6.9 | 3.8–5.4 | 93.5–97.6 | 94.4–99.8 | 97.2–99.2 | 0.999 | ||||
CerC22:0 | – | 0.005 | 1.9–3.3 | 3.9–5.9 | 95.9–98.5 | 91.2–99.6 | 98.9–101.7 | 0.999 | ||||
CerC24:0 | – | 0.005 | 1.7–6.2 | 2.9–5.4 | 96.5–98.6 | 95.1–99.9 | 99.6–100.2 | 0.999 | ||||
CerC24:1 | – | 0.005 | 1.4–4.8 | 2.6–4.7 | 95.2–99.1 | 96–99.7 | 98.5–103.2 | 0.999 | ||||
Basit et al. (27) | CerC16:0 | – | 0.00098 | 1.6–3.9 | 3.0–6.7 | 92.6–99.6 | 96.1–99.6 | LLE (BD) | CHCl3/MeOH | 87.1–89.4 | 3 | 0.991 |
CerC18:0 | – | 0.00099 | 0.5–2.6 | 2.6–7.5 | 92.5–96.9 | 93.1–98.9 | 85.9–91.1 | 0.993 | ||||
CerC22:0 | – | 0.001 | 3.2–4.2 | 2.0–7.9 | 94.5–99.7 | 95.6–97.3 | 87.1–91.1 | 0.995 | ||||
CerC24:0 | – | 0.001 | 5.3–7.8 | 0.7–11.4 | 95.8 − 100.6 | 95.5–97.3 | 87.3–89.7 | 0.995 | ||||
CerC24:1 | – | 0.00099 | 1.8–5.6 | 1.3–8.2 | 98.5 − 105.4 | 100.1 103.2 | 86.1–102.8 | 0.996 | ||||
Begou et al. (28) | CerC16:0 | 0.0013 | 0.0043 | 6.2–8.8 | 1.4–4.1 | 98.1–103 | 97.6–103 | LLE (MTBE; modified Folch) | MTBE/MeOH; CH2Cl2/MeOH | 66.1–120 | 5 | 0.999 |
CerC18:0 | 0.0012 | 0.0041 | 6.6–10.8 | 3.3–5.4 | 87.8–101 | 86.6–101.1 | 82–141 | 0.998 | ||||
CerC24:0 | 0.00063 | 0.0022 | 5.9–14.0 | 2.2–4.2 | 94–106 | 90.1–105.5 | 104–220 | 0.999 | ||||
CerC24:1 | 0.00063 | 0.0022 | 5.6–11.1 | 1.8–8.8 | 91–103 | 93–101.5 | 102–145 | 0.998 | ||||