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Table 1 Culture media and methods

From: Antiobesity and lipid-lowering effects of Bifidobacterium spp. in high fat diet-induced obese rats

Media

Main enumerated microorganisms

Incubation time (day)

Anaerobic culturea

  

BL agar mediumb

Predominant anaerobes

3

GAM agar mediumc

Bifidobacterium spp.

1-2

  1. aCulture media were prepared and dispensed into Petri dishes on a clean bench. Dishes were stored in a bactron anaerobic chamber (Sheldon Manufacturing Inc., USA) containing 90% N2, 5% CO2 and 5% H2 until plating. A sample of each dilution was plated on each medium in an anaerobic glove box. Plated dishes were incubated in a bactron anaerobic chamber filled with CO2 gas. The presence of O2 was monitored with steel wool soaked in acidified copper sulfate solution.
  2. bBL, Blood Liver (Nissui Pharm. Co. Ltd., Japan).
  3. cGAM, General Anaerobic Medium (Nissui Pharm. Co. Ltd., Japan).
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Lipids in Health and Disease

ISSN: 1476-511X