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Table 3 Kinetic parameters between stingray lipase and colipases of various species deduced from figure 4A

From: Biochemical properties of pancreatic colipase from the common stingray Dasyatis pastinaca

 

CoSPL

CoTPL

CoCPL

CoDrPL

Apparent Kd (10 -9 M)

1.2 ± 0.01

3.6 ± 0.2

3.9 ± 0.31

2.2 ± 0.15

Apparent Vmax (μmol min -1 mg -1 )

3200 ± 120

2895 ± 150

2799 ± 120

3047 ± 225

kcat (S -1 )

2400 ± 156

2171 ± 96

2242 ± 154

2539 ± 213

kcat/Kd (S -1 M -1 10 -9 )

2073 ± 95

599 ± 32

576 ± 36

1137 ± 160

  1. The apparent Kd were deduced from the slope of the linear curves of the Scatchard plots. The experiments were conducted three times.
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Lipids in Health and Disease

ISSN: 1476-511X