Biochemical properties of pancreatic colipase from the common stingray Dasyatis pastinaca
- Abir Ben Bacha†1,
- Aida Karray†1,
- Lobna Daoud1,
- Emna Bouchaala1,
- Madiha Bou Ali1,
- Youssef Gargouri1 and
- Yassine Ben Ali1Email author
© Ben Bacha et al; licensee BioMed Central Ltd. 2011
Received: 2 February 2011
Accepted: 8 May 2011
Published: 8 May 2011
Pancreatic colipase is a required co-factor for pancreatic lipase, being necessary for its activity during hydrolysis of dietary triglycerides in the presence of bile salts. In the intestine, colipase is cleaved from a precursor molecule, procolipase, through the action of trypsin. This cleavage yields a peptide called enterostatin knoswn, being produced in equimolar proportions to colipase.
In this study, colipase from the common stingray Dasyatis pastinaca (CoSPL) was purified to homogeneity. The purified colipase is not glycosylated and has an apparent molecular mass of around 10 kDa. The NH2-terminal sequencing of purified CoSPL exhibits more than 55% identity with those of mammalian, bird or marine colipases. CoSPL was found to be less effective activator of bird and mammal pancreatic lipases than for the lipase from the same specie. The apparent dissociation constant (Kd) of the colipase/lipase complex and the apparent Vmax of the colipase-activated lipase values were deduced from the linear curves of the Scatchard plots. We concluded that Stingray Pancreatic Lipase (SPL) has higher ability to interact with colipase from the same species than with the mammal or bird ones.
The fact that colipase is a universal lipase cofactor might thus be explained by a conservation of the colipase-lipase interaction site. The results obtained in the study may improve our knowledge of marine lipase/colipase.
Lipases (triacylglyceride ester hydrolase, EC 18.104.22.168) catalyze the hydrolysis of triglycerides at the lipid-water interface. In contrast to esterases which exhibit their maximal activity on soluble substrate, lipases require the presence of an interface to be fully active . It is established that the accumulation of amphiphiles at the oil/water interface in the duodenum of vertebrates (the oil-in-water droplets are covered by several natural surfactants) prevent pancreatic lipase (PL) binding.
Pancreatic colipase is a required co-factor for pancreatic lipase, being necessary for its activity during hydrolysis of dietary triglycerides in the presence of bile salts . In the intestine, colipase is cleaved from a precursor molecule, procolipase, through the action of trypsin . This cleavage yields a peptide called pentapeptide called enterostatin knoswn to regulate food intake in higher mammals, being produced in equimolar proportions to colipase [3, 4].
The understanding of PL activation and catalysis has progressed dramatically thanks to the determination of the three-dimensional structures of the uncomplexed human PL by Winkler et al.  and of a human pancreatic lipase/porcine colipase complex studied in the presence of mixed micelles by van Tilbeurgh et al. . In the uncomplexed enzyme, a large amphiphilic loop (the flap) blocks the active site access, thus explaining the limited catalytic activity of pancreatic lipase in solution. The pancreatic lipase/porcine colipase complex , in turn, has the active site exposed and the flap establishes several polar contacts with colipase. This rearrangement gives rise to an extensive hydrophobic surface , which may be involved in the interaction of the pancreatic lipase/colipase complex with tri- and diglyceride substrate.
Later, Hermoso et al., (1996) have published the crystal structure of the porcine pancreatic lipase/porcine colipase complex from crystals obtained using the non-ionic detergent tetraethylene glycol monooctyl ether (C8E4) and they found that PLhad the open conformation and attributed this result to the presence of detergent micelles in the crystallization medium. In fact, they have also observed that inhibition of PL by the serine-specific inhibitor diethyl p-nitrophenylphosphate (E600) in solution, a reaction that requires an accessible active site, only takes place in the presence of pancreatic lipase, colipase and supermicellar concentrations of either non-ionic detergents or bile-salts .
The biology and the biochemistry of mammalian colipase are well documented and several studies have provided evidence that no difference can be observed among mammals in the activation of a PL from one species by colipase from another species when emulsified triolein or tributyrin (TC4) is used as a substrate [8, 9]. However, a recent study showed that bird and mammal lipases are more activated by their own colipases. This finding can be attributable to a higher specificity of the colipase-lipase interaction .
The aquatic world contains a wide variety of living species and represents a great potential for discovering new proteins. However, in our knowledge, no colipase from the marine vertebrate have been purified so far except for the Squalus acanthius which was the first colipase purified and characterized from the dogfish pancreas .
It is therefore interesting to study some catalytic and biochemical properties of another purified marine colipase to gain more information about the marine pancreatic colipase function. This paper reports the purification to homogeneity of an active pancreatic colipase from the common stingray Dasyatis pastinaca. This colipase, tentatively named stingray pancreatic colipase (CoSPL) was characterized with respect to its biochemical properties.
Results and discussion
Purification of CoSPL and NH2-terminal sequence determination
Flow sheet of stingray pancreatic colipase purification
Total (a) activity (units)
Protein (b) (mg)
Specific activity (U/mg)
Activity recovery (%)
Heat and acidic treatment
(NH 4 ) 2 SO 4 Precipitation (50%)
Ethanol fractionation (50-90%)
Mono S Sepharose
Mono Q Sepharose
Altogether, these results suggest that marine colipase is a monomeric protein, as discribed for mammal and bird colipases.
Alignment of the N-terminal sequence of Stingray colipase with dog fish, turkey, dromedary, chicken and pig pancreatic colipases
The presence of eventual glycan chains in pure colipase molecule was investigated. Our results showed that the purified protein is not glycosylated (data not shown).
Effect of colipase on the tributyrin hydrolysis rate by pancreatic lipase
It has been established that some mammalian pancreatic lipases lack enzymatic activity when TC4 is used as substrate in the absence of bile salts and colipase. The high energy existing at the tributyrin/water interface could be responsible for their irreversible denaturation .
Activation of bile salts inhibited stingray lipase by colipases from various species
It is well established that bile salts are strong inhibitors of all pancreatic lipases independently of their origins [14, 15]. At low concentration (below CMC) bile salts stabilize lipase at interfaces . Neverless, higher concentration of bile salt inhibit the lipase activity by desorbing it from its substrate (interface).
A previous comparative study showed that mammalian colipase presents lower activation effect towards bird lipases than the bird counterpart . In order to get more information about the pancreatic lipase-colipase complex function specificity, we investigated, in this study, the activation of the bile salt-inhibited SPL by pure colipase from dromedary, turkey, chicken and stingray. Activity of pure PLagainst emulsified olive oil was determined at increasing concentrations of bile salts in the absence or in the presence of a molar excess of colipase (data not shown). NaTDC was shown to act as a strong inhibitor of pancreatic lipase. Inhibition was reversed after addition of stingray colipase to the assay system. No significant difference was observed regarding the ability of pure colipase from dromedary, chicken or turkey to activate bile-salt-inhibited SPL (data not shown).
Kinetic parameters between stingray lipase and colipases of various species deduced from figure 4A
Apparent Kd (10 -9 M)
1.2 ± 0.01
3.6 ± 0.2
3.9 ± 0.31
2.2 ± 0.15
Apparent Vmax (μmol min -1 mg -1 )
3200 ± 120
2895 ± 150
2799 ± 120
3047 ± 225
kcat (S -1 )
2400 ± 156
2171 ± 96
2242 ± 154
2539 ± 213
kcat/Kd (S -1 M -1 10 -9 )
2073 ± 95
599 ± 32
576 ± 36
1137 ± 160
Kinetic parameters between stingray colipase and lipases of various species deduced from figure 4B
apparent Kd (10-9M)
apparent Vmax (μmol min-1mg-1)
1.15 ± 0.05
3200 ± 102
2400 ± 95
2074 ± 107
2.9 ± 0.1
3655 ± 85
2741 ± 150
946 ± 48
4.5 ± 0.5
3428 ± 132
2747 ± 87
611 ± 30
3 ± 0.2
3341 ± 135
2785 ± 135
930 ± 71
This might reflect the higher ability of the marine enzyme to interact with colipase from the same species than with the mammal or bird ones when olive oil was used as substrate. This result might suggest that this difference in the affinity towards pancreatic lipases from different species might be related to the structural differences between the marine, bird and the mammal colipases.
Previous works established the fact that cofactors from different mammal species are interchangeable and fully activate mammal pancreatic lipases [18–20]. The same value of Kd (1.1 10-9 M) was obtained by Rathelot et al. when horse lipase was used, in presence of substrate, to form a complex with colipases from horse, pig or ox . Recently it has been shown that bird and mammal lipases are more activated by their own colipases .
Western blotting experiment was performed to check the immunological cross-reactivity of colipases from different species with anti-CoOPL serum. Only bird colipases reacted strongly with anti CoOPL (Figure. 1B). No cross-immunoreactivity was detected between anti-CoOPL serum and stingray or dromedary colipases. This might be explained by the fact that CoSPL surface exposed antigenic determinants are different from those present on bird pancreatic colipase surfaces.
Although the 32 residues of CoSPL NH2-terminal end showed significant homology with those of bird and mammal pancreatic colipases, the absence of immunoreactivity between CoSPL and anti-CoOPL serum strengthen the hypothesis that the amino acid exposed on the CoSPL surface, which are the main antigenic determinant, could be different from those of bird pancreatic colipase. This hypothesis needs further structural and biochemical investigations. Work is on progress in our laboratory to investigate this hypothesis.
Materials and methods
Tributyrin (99%, puriss) and benzamidine were from Fluka (Buchs, Switzerland), Triton X-100, β-mercaptoethanol (βME), bovine serum albumin (BSA) (99%, puriss), sodium taurodeoxycholate (NaTDC) was from Sigma Chemical (St. Louis, USA) and nitrocellulose membrane were from Sigma Chemical (St. Louis, USA), arabic gum was from Mayaud (Dagenham, UK), acrylamide and bis-acrylamide electrophoresis grade were from Bio-Rad (Paris, France), marker proteins and supports of chromatography used for CoSPL purification, Mono-Q Sepharose, Mono-S Sepharose were from Pharmacia (Uppsala, Sweden), PVDF membrane and protein sequencer Procise 492cLC provided from Applied Biosystems (Roissy, France), pH-stat was from Metrohm (Herisau, Switzerland).
Stingrays (Dasyatis pastinaca) pancreases were collected from a local fish market (Sfax, Tunisia) and stored at -20°C.
Enzymes and proteins
Dromedary, turkey and chicken pancreatic lipases were prepared in our laboratory as described previously . Stingray pancreatic lipases was purified in our laboratory (unpublished data). Dromedary, turkey and chicken pancreatic colipases were purified according to previous works [10, 12].
Delipidation of pancreases
After decongelation, pancreases were cut into small pieces (1-2 cm2) and delipidated according to the method described previously . After delipidation, about 15 g of delipidated powder of each pancreas were obtained from 100 g of fresh tissue.
Determination of lipase and colipase activities
Lipase activity was measured titrimetrically at pH 8.5 and 37°C with a pH-stat, under the standard assay conditions described previously, using olive oil emulsion  or TC4 (0.25 ml) in 30 ml of 2.5 mM Tris-HCl and 1 mM CaCl2, pH 8.5 , as substrate. Some lipase assays were performed in the presence or absence of NaTDC and colipase. One lipase unit corresponds to 1 μmol of fatty acid liberated per minute.
Colipase activity was measured at pH 8.5 and 37°C as described by Rathelot et al. . One colipase unit corresponds to the amount of cofactor that increases bile salt-inhibited PLactivity by 1 enzyme unit.
Determination of protein concentration
Protein concentration was determined as described previously , using bovine serum albumin (E1%1 cm = 6.7) as reference.
The presence of glycan chains in the purified cofactors was checked by the anthrone-sulfuric acid method using glucose as a standard .
Alkylation of Cys residues
The alkylation of Cys residues of colipase was performed using the technique described by Okazaki, Yamada, and Imoto . One milligram of cofactor in 1 ml of 10 mM Tris-HCl and 10 mM NaCl, pH 8.2, was denaturated in 375 μl of 8 M guanidine hydrochloride, 125 μl of 1 M Tris-HCl, 4 mM EDTA, pH 8.5, and 80 mM DTT during 30 min at 60°C. S-Pyridylethylation of cysteine residues of protein was performed by adding 4 μl of vinyl pyridine during 3 h at 25°C. The modified colipase was dialyzed against water for N-terminal sequencing.
SDS-PAGE and immunoblotting technique
Analytical polyacrylamide gel electrophoresis of proteins in the presence of sodium dodecyl sulfate (SDS-PAGE) was performed following the method of Laemmli . The proteins were stained either with Comassie brilliant blue or silver nitrate. Samples for sequencing were electroblotted according to Bergman and Jörnvall . Protein transfer was performed during 1 h at 1 mA/cm2 at room temperature.
The reactivity of anti-CoOPL serum with CoSPL, CoOPL, CoTPL and CoDrPL was checked using immunoblotting technique. After protein transfer, membranes were rinsed three times with PBS (phosphate buffer saline: 10 mM phosphate pH 7.2, 150 mM NaCl), then saturated with 3% of milk powder in PBS (saturating buffer) for 1 h at room temperature. Thereafter, anti-CoOPL serum diluted at 1:500 with PBS containing 0.05% Tween-20 (PBS/Tween-20) was incubated with the membranes for 1 h at room temperature. Afterwards, membranes were washed three times with PBS/Tween-20 then incubated for 1 h at room temperature with a 1:2000 dilution of alkaline phosphatase-conjugated anti-rabbit immunoglobulin (Sigma). After washing as mentioned above, membranes were incubated with a phosphatase substrate solution containing 0.3 mg/ml of nitroblue tetrazolium chloride (Sigma), 0.2 mg/ml of 5-bromo-4-chloro-3 indolyl-phosphate (Sigma) and 0.2 mg/ml of MgCl2 to reveal the specific immunoreactivity.
NH2-terminal sequence analysis
The NH2-terminal end of ray pancreatic colipase was sequenced by automated Edman's degradation using an Applied Biosystems Protein sequencer Procise 492 cLC .
Purification of pancreatic colipase from the stingray pancreas
Ten grams of stingray pancreas delipidated powder were suspended in 150 ml of water containing 2 mM benzamidine, 150 mM NaCl, and 0.2% Triton X-100 (v/v) and ground mechanically twice for 30 s at 4°C using the Waring Blendor system. The mixture was stirred with a magnetic bar for 30 min at 4°C and then centrifuged for 30 min at 12,000 rpm.
- Heat and acidic treatments
To inactivate the lipase, the supernatant was incubated for 5 min at 55°C. After rapid cooling, insoluble materials were removed by centrifugation for 30 min at 12,000 rpm. Afterward, the pH of the previous supernatant was brought to 2.0 by adding 6 N HCl under gentle stirring at 0°C. After centrifugation (30 min at 12,000 rpm), the clear supernatant, which was adjusted to pH 7 with 6 N NaOH, contained 7,800 colipase units per gram of delipidated pancreatic tissue.
- Ammonium sulfate precipitation
Extract from stingray pancreas was brought to 50% saturation with solid ammonium sulfate under stirring conditions and maintained for 30 min at 4°C. After centrifugation (30 min, 12,000 rpm), precipitate was resuspended in a minimum volume of extraction solution. Insoluble proteins were discarded by centrifugation (15 min, 12,000 rpm). Preparations of colipase contained of about 80% of the starting amount of colipase.
- Ethanol fractionation
Supernatant issued from ammonium sulfate precipitation was subjected to fractionation using ethanol. We added an equal volume of ethanol at 0°C. Insoluble proteins were removed by centrifugation, and the ethanol (4 v/v) was added slowly to the supernatant, bringing the solvent concentration to 90% (v/v) at 0°C. Precipitated proteins, which contained 65% of the starting amount of colipase, were collected and solubilized in minimum volume of 100 mM acetate buffer, pH 4.5, containing 0.05% Triton X-100 and 2 mM benzamidine (buffer A). Insoluble proteins were discarded by centrifugation (10 min, 12,000 rpm) and the clear supernatant thus obtained was dialyzed overnight at 4°C against buffer A.
- FPLC anion exchange Mono-S Sepharose
- FPLC Anion exchange chromatography
Dialyzed active fractions were subjected to anion-exchange chromatography using a Mono Q column (2.6 cm × 20 cm) equilibrated with buffer B. Non bound proteins were washed out with 200 ml of buffer B. After a wash with 100 ml of 0.1 M NaCl in buffer B, elution was performed with a linear gradient of NaCl (0.1-0.3 M). Stingray colipase activity emerged in a single peak at a NaCl concentration of 200 mM (figure 4B). The recovery of colipase activity after the Mono Q step was 75%. Colipase was lyophilized and conserved at -20°C.
In recent years, characterizations of enzymes from aquatic species have taken place and this had led to the emergence of new applications of these enzymes. In this study, we provided evidence that marine pancreas contains cofactors with very similar biochemical properties with those of turkey, chicken and dromedary cofactors, despite differences observed in the affinity among marine, bird and mammal colipases. Thus, marine, bird and mammal pancreatic systems appear to be functionally similar. The fact that colipase is a universal lipase cofactor might thus be explained by a conservation of the colipase-lipase interaction site. The results obtained in the study may improve our knowledge of marine lipase/colipase. In fact, the lipase/colipase system could be an interesting target to improve the control of the industrial processing of seafood during handling, chilled and frozen storage.
This work was supported by DGRST granted to the "Laboratoire de Biochimie et de Génie Enzymatique des Lipases".
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