Minor lipid components of some Acacia species: potential dietary health benefits of the unexploited seeds
© Nasri et al.; licensee BioMed Central Ltd. 2012
Received: 12 April 2012
Accepted: 11 May 2012
Published: 11 May 2012
Oilseed samples from four Acacia species ( A. cyclops, A. ligulata, A. salicina and A. cyanophylla) were analyzed in order to evaluate the potential nutritional value of their unexploited seeds.
Samples were collected from different Tunisian geographic locations. Seed oils were extracted and carotenoids, tocopherols and sterols were analyzed using chromatographic methods.
The studied Acacia seeds seem to be quite rich in lipids (from 6% to 12%). All Acacia species contain mainly the xanthophylls zeaxanthin and lutein compounds: from ca. 38 mg.kg-1 of total lipids (A. cyclops) to ca. 113 mg.kg-1 of total lipids (A. cyanophylla). Total tocopherols varied from ca. 221 mg.kg-1 of total lipids (A. cyclops) to ca. 808 mg.kg-1 of total lipids (A. ligulata). Sterols are highly present and their contents ranged between ca. 7 g. kg-1 of total lipids (A. salicina) and 11 g. kg-1 of total lipids (A. cyclops).
This study highlights that these unexploited seeds might have a potential nutritional value and encourages researchers to more explore and find developments for these plants for healthy purposes.
Currently, worldwide interest is oriented for the recovery and exploitation of oils from natural plant resources. Vegetable oils with a high relative amount of minor lipid components are of great importance for human health .
Plant sterols (phytosterols) are natural dietary components with serum cholesterol-lowering proprieties. Sterols are a group of fundamental compounds of cell membranes in both plants and animals. The most common plant sterols are β-sitosterol, campesterol, and stigmasterol, which are classified as 4- desmethylsterols of the cholestane series . The structures of plant sterols are similar to that of cholesterol with an extra methyl or ethyl group and a double bond in the side chain. Unlike cholesterol, they are not synthesized by the human body and are minimally absorbed from the gut . The exact mechanism of their cholesterol lowering properties is not fully understood, but plant sterols appear to inhibit the uptake of dietary and biliary cholesterol from the distal small intestine by competing with cholesterol for incorporation into mixed micelles . Plant sterol and stanol-enriched spreads are now widely available commercially as functional foods, but also have specific potential uses in clinical practice. Plant sterols are important ingredients of the blended functional oil . Tocopherols are considered to be the most effective lipid phase natural antioxidants. They prevent lipid peroxidation by acting as peroxyl radical scavengers that terminate chain reactions in membranes and lipoprotein particles. The role of tocopherols in cellular signaling, especially in relation to protein kinase C was also confirmed . Carotenoids are fat soluble compounds that are associated with the lipidic fractions . Carotenoids are synthesized by plants and many microorganisms. They are recognized mainly as natural antioxidants and enhancers of the immune response . Recently, these properties have increased the interest on the analysis of carotenoids in vegetable samples.
Nowadays, plant seeds constitute new oil sources, especially from underexploited seeds such as Acacia genus. Little is known about the chemistry of most Acacia species, although the genus is quite large and widespread in the warm sub-arid and arid portions of the world . The Acacia genus comprises approximately 1350 species . The present paper is to investigate, for the first time the carotenoids, tocopherols and sterols from seeds of some Tunisian Acacia species ( A. cyclops A. ligulata A. salicina and A. cyanophylla). The potential dietary importance of their unexploited seeds is discussed.
Materials and methods
Samples from fully mature fruits were collected in June 2010 and used in the present study. Acacia seeds were harvested from four species found in Tunisia, respectively A. cyclops, A. ligulata, A. salicina and A. cyanophylla.
All solvents used during the experiments (methanol, dichloromethane) were purchased from Fluka (Ridel-de Haën, Switzerland). 5α-cholestane, lutein, zeaxanthin, α-tocopherol, γ-tocopherol and δ-tocopherols were purchased from Sigma-Aldrich (Steinheim, Germany). Petroleum ether, potassium hydroxide pellets and anhydrous sodium sulphate were obtained from Merck (Darmstadt, Germany).
The oil content was determined according to the reference . About 20 g of Acacia seeds was ground in a mortar and extracted using petroleum ether in a Soxhlet apparatus for 6 h. The solvent was concentrated using a rotary evaporator under reduced pressure at 45°C. The oil was dried under a nitrogen stream and stored at −20°C until use. To minimize the decomposition and oxidation of the extracted compounds, all samples were collected in brown glass bottles to prevent UV-activated degradation. All analyses were performed in triplicate.
Extraction of lutein, zeaxanthin and tocopherols
40 mg of oil was resuspended in 1 mL of a mixed HPLC mobile phase: acetonitrile/methanol at 50 mM, and ammonium acetate/water/dichloromethane (700:150:50:100, by vol.). After resuspension, the extract was vortexed for 30 s. Samples of 80 μL were injected into the HPLC system for the analysis of lutein and zeaxanthin.
HPLC analysis of lutein, zeaxanthin and tocopherols
The HPLC apparatus was a Jasco PU-1580 Plus intelligent pump equipped with an automatic injector system AS300 (Thermo Finnigan, les Ulis, France) and a Jasco MD-1510 plus multi-wavelength detector (JASCO International Co., Ltd., Japan). HPLC analyses were carried out using RP-HPLC with a Nucleosil C18 column (25 mm x 4.6 mm id, 5 μm particle size) and a VIDAK C18 column (25 mm x 4.6 mm id, 5 μm particle size). The analytical conditions were based on those reported by [Lyan et al. 12], with some modifications: Isocratic solvent system; acetonitrile/methanol at 50 mM ammonium acetate/water/dichloromethane (700:150:50:100, by vol.); flow rate = 2 mL.min-1, and detection at 450 nm for lutein and zeaxanthin and 298 nm for tocopherols.
Identification of lutein and zeaxanthin
The identification of lutein, zeaxanthin and tocopherols was ensured by comparing the retention times and absorption spectra of unknown peaks with those of reference standards and by adding lutein, zeaxanthin, α-, β-, δ-tocopherol standards to the sample for co-chromatography.
Preparation of the standard curve
Six quantities of lutein (range: 0.125–5 μg), zeaxanthin (range: 50–300 ng), α-tocopherol (range: 0.5–40 μg), γ-tocopherol (range: 1–25 μg) and δ-tocopherol (range: 0.1–2 μg) were injected into the HPLC system (each standard being dissolved in 1 mL of the HPLC mobile phase: acetonitrile/methanol at 50 mM ammonium acetate/water/dichloromethane (700:150:50:100, by vol.). The linear regression equation for each standard curve was then obtained by plotting the amount of the standard compound injected against the peak surface area. The regression equation and correlation coefficient (r2) were calculated using ChromNav software (JASCO).
A mixture of 50 mg of seed oil, 25 μL 5α cholestane (1 mg.mL-1) used as an internal standard and 5 mL methanolic KOH (1 N) was saponified in a capped flask for 16 h at room temperature. 10 mL distilled water and 10 mL dichloromethane were then added and mixed. The resulting solution was centrifuged and the lower fraction was kept in a second capped flask. The upper organic layers were washed twice with 10 mL distilled water, and once with 10 mL dichloromethane. The resulting solution was centrifuged and the dichloromethane layers were combined and washed twice with 5 mL and kept in the second capped flask. This solution was filtered and the obtained solvent was evaporated to dryness under nitrogen at 40°C. After vortexing, the aliquot (matter unsaponifiable with dichloromethane) was derivatized to trimethylsilyl ethers (TMS ether) by the addition of 300 μL N,O-bis(trimethylsilyl)trifluoroacetamide and 50 μL pyridine at 60°C for 30 min, and then injected into the gas chromatograph.
Sterol quantification by gas chromatography-flame ionization detection (GC–FID)
Samples (2 μL) were analyzed in duplicate by GC in a Hewlett-Packard HP-4890D chromatograph equipped with a 30 m (0.25 mm i.d., 0.25-μm film thickness) DB5 MS fused silica capillary column. The oven temperature was raised from 50°C to 290°C at a rate of 20°C min-1. The flame ionization detector (FID) temperature was 290°C. The split ratio was 1:20. Helium was used as a carrier gas at a pressure of 120 kPa. TMS esters were eluted from the column. The data were processed using EZChrom Elite software (Agilent Technologies, Massy, France). The areas of both sterols were compared to the areas of known quantities of the internal standard (5α-cholestan).
Sterol identification by gas chromatography–mass spectrometry (GC-MS)
GC-MS analyses of TMS ester derivatives were carried out on a Shimadzu GC 2010 gas chromatograph attached to a Shimatdzu 2010 selective quadrupole mass detector (Shimadzu France, Marne la Vallée) operating in the electronic ionisation mode under an ionisation voltage of 70 eV at 200°C. Shimadzu software was used for data acquisition and processing. The injector (splitless mode) and the interface temperature were maintained at 290°C; helium was used as the carrier gas under a constant flow rate of 1 mL/min. Spectral data were acquired over a mass range of 50–600 amu. GC separation was performed on a DB5 MS fused silica capillary column (0.25 mm i.d., 0.25-μm film thickness). The temperature was kept at 50°C for 1 min, and then raised to 290°C for 90 min at a rate of 20°C min-1.
Statistical and chemometric methods
Data were compared on the basis of standard deviations from mean values. Differences between mean values were based on the one-way analysis of variance with a post-hoc determination using Duncan’s multiple range tests performed by Statistica software (version 8). The level of significance was set at p <0.05.
Results and discussion
Carotenoids and tocopherols † (mg.kg -1 of total lipids) of Acacia seed oils
Oil content (dw%)
Carotenoid and tocopherol contents
All Acacia species contain mainly the xanthophylls zeaxanthin and lutein compounds as reported in Table 1. Total amounts of xanthophylls were respectively for A. cyclops, A. ligulata, A. salicina and A. cyanophylla: 38.21 mg.kg-1 TL (Total Lipids), 50.10 mg.kg-1 TL, 57.04 mg.kg-1 TL, and 113.76 mg.kg-1 TL. Contents of the main compound (lutein) were 35.41 mg.kg-1 TL (A. cyclops), 43.83 mg.kg-1 TL (A. ligulata), 54.67 mg.kg-1 TL (A. salicina) and 109.55 mg.kg-1 TL (A. cyanophylla). These differences are mainly due to genetic factors. Highly amounts of carotenoids make the genus Acacia a good natural source of these compounds, especially luteins.
As for tocopherols, all Acacia species contain mainly α- and γ-tocopherols and in some few amounts the δ-tocopherol. Table 1. The absence of ß-tocopherol was confirmed using the same method previously described. Regarding α and γ tocopherol contents for the different studied Acacia, we noticed that α-tocopherol is the major compound for A. salicina (404.30 mg.kg-1 TL) and A. cyanophylla (560.14 mg.kg-1 TL). As for as A. cyclops and A. ligulata, γ-tocopherols was found to be the major compound, with respectively 127.25 mg.kg-1 TL and 462.14 mg.kg-1 TL. For all Acacia, the total tocopherols ranged between 221.42 mg.kg-1TL (Acacia cyclops) and 808.76 mg.kg-1 TL (Acacia ligulata). Tocopherols from all studied Acacia were highly presented compared to some other vegetable oils like grape seed (142.6 mg.kg-1 TL) and are comparable to those of olive (216.8 mg.kg-1), flaxseed (588.5 mg.kg-1), peanut (398.6 mg.kg-1), pumpkin (508.1 mg.kg-1 TL), rapeseed (624.6 mg.kg-1 TL), and sunflower (634.4 mg.kg-1 TL). But these contents are lower than soybean (1797.6 mg.kg-1 TL) or maize (1618.4 mg.kg-1 TL), for example .
Sterols (g. kg -1 of total lipids) of Acacia seed oils
Δ 5,23 stigmastadienol
Δ 5, 24 (25) stigmasterol
Δ5, 24 stigmastadienol
24, methylene cycloartanol
The studied Acacia s seem to be quite rich in lipids (from 6 to 12%) and are well comparable to other Acacia species. The composition of Acacia species lipid fraction is reported here for the first time. Studied Acacia species contain very high levels of carotenoids, tocopherols and sterols. Carotenoids from studied Acacias reached 113 mg.kg-1 TL and tocopherols reached 808 mg.kg-1 TL. Sterols reached 11 g.kg-1 TL. As these minor compounds are known to have a wide range of beneficial biological activities and physical properties, the oil from Acacia seeds confirms its nutritional value and dietary importance. This study explores that these unexploited seeds might replace conventional oil types such sunflower or rapeseed oils.
The authors would like to thank the ChemoSens platform (CSGA, INRA- Dijon, France) for its technical support with respect to GC-MS.
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